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Technical University of Munich
Technical University of Munich
(a) Magnetic fingerprints of immunomagnetically labeled single cells under laminar flow conditions are acquired by a Wheatstone half-bridge layout with two GMR resistors.(b) Cell positions are allocated to simulated and measured characteristic signal propagation. (c) In situ magnetophoretic cell enrichment and focusing on NiFe patterns allows for highest lateral reproducibility of the magnetically labeled targets. Scale bar is 100 μm. (d) Magnetic signal pattern allows for effortless calculation of cell characteristics from the magnetic fingerprint. Adopted from Reisbeck et al., Magnetic fingerprints of rolling cells for quantitative flow cytometry in whole blood, Sci. Rep. 6, 32838 (2016).

(a) Sensor integration into credit-card sized moulded cartridge. (b,c,d) Functional package with mechanical and magnetophoretic particle anlignment. (e) Sensor transfer curve for µT magnetic field detection of immunomagnetically labeled cells. Adopted from Reisbeck et al., Hybrid integration of scalable mechanical and magnetophoretic focusing for magnetic flow cytometry, Biosens. Bioelectron. 109, 98-108 (2018).

Magnetic Flow Cytometry

We have developed magnetic biosensors incorporating magnetophoretic single cell manipulation and in-flow detection of biological specimen by magnetoresistive sensor devices. Mimicking cell rolling, as found in endothelial vessels, our approach allows us to perform highly specific and sensitive single cell characterization.

Quantitative volumetric information, immunomagnetic binding capacity, and morphological parameters can be obtained on a single cell level even in complex biological matrices. Thus, our integrated approach allows for minimized sample preparation effort and analysis in a native cellular environment such as unprocessed whole blood.

We further work on the workflow integration of biosensors and microfluidics in total analytical systems. In this way we achieve the translation of Lab-on-a-Chip platforms to the preclinical and clinical applications. We have developed an adaptive and scalable particle enrichment concept to overcome the spatial resolutions of biosensors.

 

Literature and topological reviews:

Magnetic fingerprints of rolling cells

Hybrid integration for magnetic flow cytometry

Magnetic diagnostic systems

Microsystems for single-cell analysis

 

Contact: Moritz Leuthner, moritz.leuthner@tum.de

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