Optimization of detection on malathion using single strand DNA
Introduction
- Detection of small molecules can be challenging. One of the methods to detect them is using aptamers, which are artificially synthesized single strands of DNA, selected specifically for detection of a certain analyte
- This project aims to examine detection of malathion using malathion aptamer
Aim & Research Methods
Your task is to…
- Fabricate test electrodes (on flexible or rigid substrate)
- Incubate aptamers on the electrodes with or without filling molecules
- Characterize binding affinity using SPR
- Explore detection of malathion via electrochemical methods
You will be introduced in an interdisciplinary working environment and will learn the following techniques:
- techniques for rapid prototyping such as laser-patterning
- electrochemical characterization of functionalized electrodes using potentiostat
- experimental design
- use of micropipetting robot
- Circular Dichroism Spectroscopy, Surface plasmon resonance
Requirements
- dedication and motivation to work on an interdisciplinary research topic self-reliantly
- wet-lab experience
- preferable knowledge in chemistry and electrochemistry and experience with aptamers
Possible starting date & further information
Potential starting date is February/March. For further details and application contact Marta Nikic (marta.nikic@tum.de)
Additional information
| Address: Munich Institute of Biomedical Engineering | Mail:
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